A group of indian scientists has developed a new salttolerant transgenic rice plant by overexpressing a gene from a wild rice called porteresia coarctata. Usda, ars, germplasm resources information network. Porteresia coarctata, the only halophyte wild rice, is a rich source of genes and. Pdf characterization of progenies from intergeneric. To change the order of your pdfs, drag and drop the files as you want. Draft genome sequence of first monocothalophytic species oryza. Effect of salinity on nutritional value of saltmarsh grass. Plant promoter prediction tool and isolation of a promoter. The presence of pinitol synthesizing proteingene in a wild halophytic rice is remarkable, although its exact role in salt tolerance of p. A gene coding for the chloroplastic fructose 1,6bisphosphatase pccfr in porteresia coarctata tateoka roxb. If this is the first time you use this feature, you will be asked to authorise cambridge core to connect with your account. Tateoka desiccation intolerant, recalcitrant, despite the fact that o. Porteresia coarctata is a species of grass in the family poaceae, native to india, sri lanka, bangladesh, and myanmar porteresia coarctata is considered by some botanists to be the only species in the genus porteresia.
We sequenced the transcriptome of porteresia under different conditions using illumina platform and generated about 375 million highquality reads. Porteresia coarctata syn oryza coarctata is a tetraploid wild rice growing abundantly in the coastal region of india and some other asian countries. The filtered fastq files were further subjected to more stringent. Phylogenetic analyses based on 16s rrna gene sequences indicated that the novel strains were. Intergeneric somatic hybrids of rice oryza sativa l. Two facultatively anaerobic, nitrogenfixing bacteria strains mssrf30t and mssrf31 were isolated from a mangroveassociated wild rice porteresia coarctata tateoka. Porteresia coarctata is a mangrove rice, which being wild. A novel salttolerant lmyoinositol1phosphate synthase. Scientists develop salt tolerant rice variety the hindu. Once you merge pdfs, you can send them directly to your email or download the file to our computer and view. We sequenced the transcriptome of porteresia under different conditions using illumina platform and generated about 375 million high. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. Mangroves of mahanadi delta in the state of odisha and aspects of their conservation prasanna kumar nayak1, raj kishore mohanta2.
Salt tolerance in the halophytic wild rice, porteresia. Pdf the possible overlap between salinity and mechanical. Micropropagation of porteresia coarctata tateoka, a wild relative of rice with useful genetic traits of salinity and submergence tolerance, was achieved t. To understand the mechanism of salttolerance of the wild halophytic rice, porteresia coarctata in contrast to the saltsensitive domesticated rice oryza sativa, we have compared p. Deep transcriptome sequencing of wild halophyte rice, porteresia. Notable herbaceous elements such as suaeda maritima, suaeda nudiflora, salicornia brachiata, tylophora tenuisima. Functional screening of genes from a halophyte wild rice. It is noteworthy that porteresia coarctata is closely related to oryza species, suggesting that it should be treated as a member in the genus oryza rather than a separate monotypic genus. Micropropagation of porteresia coarctata tateoka, a wild relative of rice with useful genetic traits of salinity and submergence tolerance, was.
In silico analysis confirms the absence of an imt1 homolog in oryza genome, and pcimt1 is. Pdf in nature, certain abiotic stresses constitutively acting on plants often go. Agriculture and biodiversity international union for. Pdf in vitro propagation of salttolerant wild rice relative.
Rearrange individual pages or entire files in the desired order. Pdf cloning, characterization and expression of a chloroplastic. Porteresia coarctata is a species of grass in the family poaceae, native to india, sri lanka. Variations in the protein content of porteresia coarctata may be due to difference in habitat and season14. Samples were collected during the period of low tide from the bakkhali estuary at two stations. Phylogeny of rice genomes with emphasis on origins. Effect of salinity on nutritional value of saltmarsh grass porteresia coarctata from gangetic delta, northeast coast of india. In vitro propagation of salttolerant wild rice relative, porteresia. While members of the genus oryza are very sensitive to salinity, salt concentrations as high as 20% of that of seawater had no adverse effect on the growth of the tropical wild rice porteresia coarctata tateoka in experiments undertaken in a greenhouse in the uk.
Mangroves are characterized by alkaline soils in which the halophytic wild rice relative porteresia coarctata thrives. Salt tolerant transgenic rice variety will be develop that ultimately benefits the farmers. Spatiotemporal variation of stored carbon in porteresia coarctata along the east and west coasts of india 1. Pcino1 and pcimt1, earlier isolated from porteresia coarctata were cloned into pcambia1 fig. Department of agriculture agricultural research service. Isolation of a cdna clone pcsrp encoding serinerich. Inositol methyl tranferase from a halophytic wild rice. Northern analysis and in situ mrna hybridization re vealed the expression of pcsrp in the salt stressed roots and rhizome of p. A 37amino acid stretch between trp174 and ser210 has been confirmed. A phylogeny of the rice tribe oryzeae poaceae based on.
A cdna for porteresia coarctata inositol methyl transferase 1 pcimt1, coding for the inositol methyl transferase implicated in the synthesis of pinitol has been cloned from p. An international journal for rapid publication of reports on genes and genomes, volume 21. Functional screening of genes from a halophyte wild rice relative. Chloroplastic fructose1,6bis phosphatase, porteresia coarctata eu371994 iii. Porteresia coarctata oryza coarctata is a wild halophyte, which shows. Recalcitrant seed storage physiology in three aquatic grasses zizania palustris, spartina anglica and porteresia coarctata r.
Tateoka is an endemic halophyte growing all over the coastal belt of bangladesh, propagating through rhizomes and setting a few rice. Further details about the rice species and related genera are given in tables 11 and 12. Haworthiopsis coarctata, formerly haworthia coarctata, is a species of flowering succulent plant from the eastern cape province, south africa and naturalized in mexico. Tateoka pcino1, af412340 earlier reported from this laboratory, has been analyzed by in vitro mutant and hybrid generation and subsequent biochemical and biophysical studies of the recombinant proteins. The distinct morphology and leaf architecture enabling the plant to exclude salt is a characteristic feature of porteresia in comparison. Pdf merge combine pdf files free tool to merge pdf online. Myriostachya wightiana and porteresia coarctata in muddy flats. The rice tribe, oryzeae, consists of 12 genera and is distributed in tropical and temperate regions worldwide clayton and renvoize, 1986. Total rna was isolated from mature leaves of oryza and porteresia following the method of ostrem et al. Plant promoter prediction tool and isolation of a promoter from porteresia coarctata mahalakshmi s1,krishna nc 2,rao kv 1,reddy vd 1 1 centre for plant molecular biology, osmania university, hyderabad, india and 2 sr software, jubilee hills, hyderabad, india abstract in silico identification of promoters is complicated by. These strains were determined to be nitrogenfixers using the acetylene reduction assay and by pcr detection of a nifh gene amplicon. The enhanced synthesis of pinitol in porteresia under stress may.
Selective manipulation of the inositol metabolic pathway for. Porteresia coarctata is a wild relative of rice with capability of high salinity and submergence tolerance. Antifeeding and toxic effects of chemical extracts from porteresia coarctata takeoka on the insect pest spodoptera litura f. Characterization of porteresia coarctata beds along the goa. Once files have been uploaded to our system, change the order of your pdf documents. Longterm exposure to combined treatment of elevated co2. Porteresia coarctata, the only halophyte wild rice, is a rich source of genes and promoters for improving salt tolerance in rice. The habitat of porteresia coarctata from mandovi estuary, goa. Using illumina and nanopore reads, we achieved the assembled genome size of 569. Regulatory region of ubiquitin 2 gene from porteresia coarctata makes efficient promoters for transgene expression in monocots and dicots. The salt tolerance property of this mangrove associate has been dealt with by a number of workers earlier. Porteresia coarctata phosphoenolpyruvate carboxylase eu371116 v. Seed development, the accumulation of abscisic acid and.
Pdf a gene coding for the chloroplastic fructose 1,6bisphosphatase pccfr in porteresia coarctata tateoka roxb. Problems and prospects for utilizing porteresia coarctata in rice breeding programs created date. Porteresia coarctata oryza coarctata is a relative of oryza rice found in mangrove areas. Estuarine macrophytes at bakkhali, coxs bazar, bangladesh. Deep transcriptome sequencing of wild halophyte rice. Other authors maintain instead that it should belong in the rice genus oryza, as oryza coarctata roxb. Salinity poses a serious threat to yield performance of cultivated rice in south asian countries. Porteresia coarctata, forms an important constituent in the plant succession leading to formation of mangrove communities along. Please, select more pdf files by clicking again on select pdf files. Merge pdf files combine pdfs in the order you want with the easiest pdf merger available. Oryza coarctata in the germplasm resources information network grin, u. Porteresia coarctata is a c4 grass which occupies a significant part of estuarine vegetation as mangrove associates in the eastern and western coasts of india.
Effects of partial drying on seed germination in the aquatic grasses zizania palustris l. It is one of the species of haworthiopsis that is commonly cultivated as an ornamental description. Oryza coarctata also known as porteresia coarctata, lu and ge, 2003 is an allotetraploid wild rice with extreme salt and submergence tolerance. Full length cdna overexpression foxcox system was used to identify porteresia coarctata genes with positive involvement in salt stress response using arabidopsis model. Recalcitrant seed storage physiology in three aquatic. The molecular basis of salt tolerance of lmyoinositol 1p synthase mips. Then, based on his studies, tateoka 1963a,b, 1965 erected a new genus, porteresia, and transferred o. Porteresia coarctata triosephosphate isomerase pctpi mrna, complete cds. The transcriptome analyses of porteresia can lead to the identification of candidate genes involved in salinity and submergence tolerance. It is a wild relative of rice which can survive salinity up to 3040 dsm ref. It is a form of wild rice that grows in saline estuaries in bangladesh. A concentration of 1,000 and 2,000 ppm extract exhibited significant. It is unique among wild rice species, since it has.
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